Incubation:Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States) or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4°C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa Fluor TM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4°C.
Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States) or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4°C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa FluorTM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4°C.
Article Title: Characterization of a novel zebrafish model of MTMR5 -associated Charcot-Marie-Tooth disease type 4B3
Article Snippet: .. Samples were incubated with primary antibodies: mouse anti-myosin (1:10, A4.1025, DSHB), mouse anti-synaptotagmin 2 (1:10, Znp-1, DSHB), or mouse anti-acetyl-tubulin (1:200, T7451, Sigma) overnight at 4°C. .. Samples were incubated with primary antibodies: mouse anti-myosin (1:10, A4.1025, DSHB), mouse anti-synaptotagmin 2 (1:10, Znp-1, DSHB), or mouse anti-acetyl-tubulin (1:200, T7451, Sigma) overnight at 4°C.
Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles.
Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1 h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4 °C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa FluorTM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4 °C.
Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1 h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4 °C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa Fluor 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4 °C.
Blocking Assay:Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States) or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4°C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa Fluor TM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4°C.
Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States) or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4°C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa FluorTM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4°C.
Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles.
Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1 h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4 °C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa FluorTM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4 °C.
Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1 h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4 °C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa Fluor 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4 °C.
other:Article Title: Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines
Article Snippet: When necessary, Prolong Diamond Antifade Mounting Media (ThermoFisher #P36970) replaced Vectashield mounting media to minimize quenching of AlexaFluor 647-conjugated antibodies.
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