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mouse anti syt2  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse anti syt2
    Mouse Anti Syt2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+synaptotagmin+2/anti-Synaptotagmin+2/pmc12530179-3-0-3
    Average 95 stars, based on 153 article reviews
    mouse anti syt2 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
    Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States) or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4°C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa Fluor TM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4°C.

    Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
    Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States) or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4°C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa FluorTM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4°C.

    Article Title: Characterization of a novel zebrafish model of MTMR5 -associated Charcot-Marie-Tooth disease type 4B3
    Article Snippet: .. Samples were incubated with primary antibodies: mouse anti-myosin (1:10, A4.1025, DSHB), mouse anti-synaptotagmin 2 (1:10, Znp-1, DSHB), or mouse anti-acetyl-tubulin (1:200, T7451, Sigma) overnight at 4°C. .. Samples were incubated with primary antibodies: mouse anti-myosin (1:10, A4.1025, DSHB), mouse anti-synaptotagmin 2 (1:10, Znp-1, DSHB), or mouse anti-acetyl-tubulin (1:200, T7451, Sigma) overnight at 4°C.

    Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles.
    Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1 h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4 °C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa FluorTM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4 °C.

    Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
    Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1 h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4 °C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa Fluor 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4 °C.

    Blocking Assay:

    Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
    Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States) or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4°C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa Fluor TM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4°C.

    Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
    Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States) or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4°C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa FluorTM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4°C.

    Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles.
    Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1 h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4 °C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa FluorTM 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4 °C.

    Article Title: Variant-specific pathophysiological mechanisms of AFF3 differently influence transcriptome profiles
    Article Snippet: .. Embryos were then incubated in blocking buffer (1% BSA in 1X PBS) for 1 h at RT and incubated in primary antibodies, mouse anti-synaptotagmin 2 (Znp-1, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), or mouse anti-islet 1 and 2 (39.4D5, diluted 1:100 in blocking solution – DSHB, Iowa City, IA, United States), overnight at 4 °C on a slow shaker. .. After 3 washes in 1X PBS, the embryos were incubated with a secondary antibody, Alexa Fluor 488 conjugated (diluted 1:500 in blocking solution, Invitrogen), overnight at 4 °C.

    other:

    Article Title: Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines
    Article Snippet: When necessary, Prolong Diamond Antifade Mounting Media (ThermoFisher #P36970) replaced Vectashield mounting media to minimize quenching of AlexaFluor 647-conjugated antibodies.



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    ( A ) Example reciprocally coupled MTC–FSI pair filled with LY and NB. ( B ) Magnified region from A , showing single optical plane ( B ). ( C ) MTC-to-FSI uEPSPs ( n = 14 event waveforms) exhibited comparable rise times as sEPSPs ( n = 3,965 event waveforms) in the pair in A and B (left; p = 0.8, r.s. = 7889511, Wilcoxon rank-sum test with Bonferroni-corrected significance level of 0.05/78), while FSI-to-MTC uIPSCs ( n = 23 event waveforms) exhibited faster rise times than sIPSCs ( n = 613 event waveforms) (right; *** p = 5.3 × 10 –11 , r.s. = 200918.5, Wilcoxon rank-sum test with Bonferroni-corrected significance level of 0.05/52). ( D ) Comparison of unitary and spontaneous synaptic event kinetics as in C (only median rise times shown for visual clarity) for 78 pairs exhibiting unitary MTC-to-FSI excitation (left) and 52 pairs exhibiting unitary FSI-to-MTC inhibition (right). ( E, F ) Single optical confocal planes of layer 5 (L5) primary visual neocortex ( E ) and hippocampal CA1 ( F ) revealing colocalization of Syb1 and PV in basket-like outlines of putative pyramidal cells. ( G, H ) Single optical confocal plane ( G ) and maximum-intensity projection (approximately 50 μm depth) ( H ) of Syb1 and PV in the OB, revealing a consistent absence of basket-like outlines of MTCs. ( I–L ) Same as E–H for <t>Syt2</t> and PV. ( M–T ) Same as E–H for PV and Cre-dependent expression of either cytosolic tdTomato ( M–P ) or membrane-localized mCitrine ( Q–T ) in PV-IRES-Cre mice. Source data for panels C and D are provided in Supporting information, . FSI, fast-spiking interneuron; LY, Lucifer Yellow; MTC, mitral and tufted cell; OB, olfactory bulb; PV, parvalbumin; sIPSC, spontaneous IPSC.
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    ( A ) Example reciprocally coupled MTC–FSI pair filled with LY and NB. ( B ) Magnified region from A , showing single optical plane ( B ). ( C ) MTC-to-FSI uEPSPs ( n = 14 event waveforms) exhibited comparable rise times as sEPSPs ( n = 3,965 event waveforms) in the pair in A and B (left; p = 0.8, r.s. = 7889511, Wilcoxon rank-sum test with Bonferroni-corrected significance level of 0.05/78), while FSI-to-MTC uIPSCs ( n = 23 event waveforms) exhibited faster rise times than sIPSCs ( n = 613 event waveforms) (right; *** p = 5.3 × 10 –11 , r.s. = 200918.5, Wilcoxon rank-sum test with Bonferroni-corrected significance level of 0.05/52). ( D ) Comparison of unitary and spontaneous synaptic event kinetics as in C (only median rise times shown for visual clarity) for 78 pairs exhibiting unitary MTC-to-FSI excitation (left) and 52 pairs exhibiting unitary FSI-to-MTC inhibition (right). ( E, F ) Single optical confocal planes of layer 5 (L5) primary visual neocortex ( E ) and hippocampal CA1 ( F ) revealing colocalization of Syb1 and PV in basket-like outlines of putative pyramidal cells. ( G, H ) Single optical confocal plane ( G ) and maximum-intensity projection (approximately 50 μm depth) ( H ) of Syb1 and PV in the OB, revealing a consistent absence of basket-like outlines of MTCs. ( I–L ) Same as E–H for <t>Syt2</t> and PV. ( M–T ) Same as E–H for PV and Cre-dependent expression of either cytosolic tdTomato ( M–P ) or membrane-localized mCitrine ( Q–T ) in PV-IRES-Cre mice. Source data for panels C and D are provided in Supporting information, . FSI, fast-spiking interneuron; LY, Lucifer Yellow; MTC, mitral and tufted cell; OB, olfactory bulb; PV, parvalbumin; sIPSC, spontaneous IPSC.
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    Image Search Results


    ( A ) Example reciprocally coupled MTC–FSI pair filled with LY and NB. ( B ) Magnified region from A , showing single optical plane ( B ). ( C ) MTC-to-FSI uEPSPs ( n = 14 event waveforms) exhibited comparable rise times as sEPSPs ( n = 3,965 event waveforms) in the pair in A and B (left; p = 0.8, r.s. = 7889511, Wilcoxon rank-sum test with Bonferroni-corrected significance level of 0.05/78), while FSI-to-MTC uIPSCs ( n = 23 event waveforms) exhibited faster rise times than sIPSCs ( n = 613 event waveforms) (right; *** p = 5.3 × 10 –11 , r.s. = 200918.5, Wilcoxon rank-sum test with Bonferroni-corrected significance level of 0.05/52). ( D ) Comparison of unitary and spontaneous synaptic event kinetics as in C (only median rise times shown for visual clarity) for 78 pairs exhibiting unitary MTC-to-FSI excitation (left) and 52 pairs exhibiting unitary FSI-to-MTC inhibition (right). ( E, F ) Single optical confocal planes of layer 5 (L5) primary visual neocortex ( E ) and hippocampal CA1 ( F ) revealing colocalization of Syb1 and PV in basket-like outlines of putative pyramidal cells. ( G, H ) Single optical confocal plane ( G ) and maximum-intensity projection (approximately 50 μm depth) ( H ) of Syb1 and PV in the OB, revealing a consistent absence of basket-like outlines of MTCs. ( I–L ) Same as E–H for Syt2 and PV. ( M–T ) Same as E–H for PV and Cre-dependent expression of either cytosolic tdTomato ( M–P ) or membrane-localized mCitrine ( Q–T ) in PV-IRES-Cre mice. Source data for panels C and D are provided in Supporting information, . FSI, fast-spiking interneuron; LY, Lucifer Yellow; MTC, mitral and tufted cell; OB, olfactory bulb; PV, parvalbumin; sIPSC, spontaneous IPSC.

    Journal: PLOS Biology

    Article Title: Fast-spiking interneuron detonation drives high-fidelity inhibition in the olfactory bulb

    doi: 10.1371/journal.pbio.3002660

    Figure Lengend Snippet: ( A ) Example reciprocally coupled MTC–FSI pair filled with LY and NB. ( B ) Magnified region from A , showing single optical plane ( B ). ( C ) MTC-to-FSI uEPSPs ( n = 14 event waveforms) exhibited comparable rise times as sEPSPs ( n = 3,965 event waveforms) in the pair in A and B (left; p = 0.8, r.s. = 7889511, Wilcoxon rank-sum test with Bonferroni-corrected significance level of 0.05/78), while FSI-to-MTC uIPSCs ( n = 23 event waveforms) exhibited faster rise times than sIPSCs ( n = 613 event waveforms) (right; *** p = 5.3 × 10 –11 , r.s. = 200918.5, Wilcoxon rank-sum test with Bonferroni-corrected significance level of 0.05/52). ( D ) Comparison of unitary and spontaneous synaptic event kinetics as in C (only median rise times shown for visual clarity) for 78 pairs exhibiting unitary MTC-to-FSI excitation (left) and 52 pairs exhibiting unitary FSI-to-MTC inhibition (right). ( E, F ) Single optical confocal planes of layer 5 (L5) primary visual neocortex ( E ) and hippocampal CA1 ( F ) revealing colocalization of Syb1 and PV in basket-like outlines of putative pyramidal cells. ( G, H ) Single optical confocal plane ( G ) and maximum-intensity projection (approximately 50 μm depth) ( H ) of Syb1 and PV in the OB, revealing a consistent absence of basket-like outlines of MTCs. ( I–L ) Same as E–H for Syt2 and PV. ( M–T ) Same as E–H for PV and Cre-dependent expression of either cytosolic tdTomato ( M–P ) or membrane-localized mCitrine ( Q–T ) in PV-IRES-Cre mice. Source data for panels C and D are provided in Supporting information, . FSI, fast-spiking interneuron; LY, Lucifer Yellow; MTC, mitral and tufted cell; OB, olfactory bulb; PV, parvalbumin; sIPSC, spontaneous IPSC.

    Article Snippet: Free-floating 50-μm sagittal sections were then incubated in blocking solution (2% normal serum and 0.1% Triton X-100, in PB), washed, and incubated in antibody solution (2% normal serum and 0.05% Tween 20, in PB) containing combinations of the following primary antibodies: guinea pig anti-parvalbumin (1:2,000; Synaptic Systems, 195 004), mouse anti-synaptotagmin-2 (1:200; Zebrafish International Resource Center, znp-1), rabbit anti-TRIM-46 (1:1,000; Synaptic Systems, 377 008), rabbit anti-Lucifer Yellow (1:1,000; Thermo Fisher Scientific, A-5750), rabbit anti-parvalbumin (1:2,000; Synaptic Systems, 195 002), rabbit anti-synaptobrevin-1 (1:500; Synaptic Systems, 104 002), rabbit anti-vasoactive intestinal peptide (1:4,000; ImmunoStar, 20077), and sheep anti-tyrosine hydroxylase (1:1,000; Millipore, AB1542).

    Techniques: Comparison, Inhibition, Expressing, Membrane